Plant parts like stem bark, leaves and fruits of Ailanthus excelsa Seem were extracted with different solvents such as methanol, ethyl acetate, alcohol, acetone, and water. The antimicrobial assay of A. excelsa extracts were evaluated on bacterial and fungal strains like Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans. Vibrio cholerae and Salmonella typhi. Phytochemical screening was performed for alkaloids, terpenoids, tannins, saponins, steroids, cardiac glycosides and flavonoids. Various solvent extracts were examined using agar disk diffusion method against bacterial and fungal microorganisms.