Standardization of phytolean™ powder a phytogenic feed supplement for lean meat production

Nowadays Ayurvedic herbal formulations have gained acceptance and demand globally due to their safe consumption and minimal side effects. This has gained acceptance in the veterinary market in recent years. This rise in the use of herbal products has also given rise to various adulteration, so it become necessary to develop standardization protocols for safety, efficacy, and quality control. These protocols must have reliable, specific, and sensitive quality control methods. Phytolean™ Powder is a herbal formulation for lean meat production containing herbs like Curcuma longa and Trigonella foenum graecum, as prime ingredients. The present study was undertaken to develop standardization parameters for Phytolean™ Powder. Reverse-phase high-performance liquid chromatography equipped with a photodiode array detector (RP-HPLC-PDA) was employed quantification of dominant biomarkers in this herbal formulation. The RP-HPLC-PDA Rt-value of Trigonelline 4.85 and Curcuminoids i.e., Curcumin, Bisdemethoxycurcumin, Demethoxycurcumin are 22.50±0.15, 24.43±0.03, and 27.15±0.05 minutes in the formulation and reference standards were found comparable under UV light at 265 nm and 420 nm respectively. The percentage of Trigonelline and Curcuminoids were 0.120±0.85 % and 0.220±0.92 % present in the Phytolean™ Powder formulation. Bioactive markers are characteristic of the ingredients or botanicals to identify the presence of ingredients in formulation easily. The quantification of biomarkers by HPLC is the best way to identify and evaluate the quality of the finished formulation in the course of the development of a standardization protocol for quality control of Ayurvedic formulation.