Dengue is one of the major public health threats in India. Every year, blood samples with dengue-like illness are referred to us from different medical colleges and hospitals in Uttar Pradesh for the detection of dengue infection in them by PCR. A total of 198 samples were referred to us for that purpose. All the samples were tested for the detection of IgG antibodies and by antigen by ELISA method, followed by RT-PCR test for the detection of serotypes. 72 samples were ELISA positive. Out of 32 antigen positive samples, all were RT-PCR positive. None of the antibody positive samples were positive by PCR. All were typed as Dengue 3 by in-house PCR.