In vitro regeneration of dalbergia sissoo roxb. from callus of cotyledonary explants

The in vitro studies with Dalbergia sissoo showed that the callus raised from cotyledonary explants had only the potential for shoot regeneration rather than the callus raised from nodal explants in provided medium. Best callus induction was observed on MS supplemented with 4.0 mg/l BAP, 0.1 mg/l NAA and 3% sucrose through nodal explants, but callus induction in cotyledonary explants was showed on 2.0 mg/l 2, 4-D, 2.0 mg/l 2, 4-D and 0.05 mg/l Kn. Callus of both explants were subcultured 3-4 time on 4.0 mg/l BAP and 0.1 NAA for multiplication and to increase the compactness of callus. Further callus of both the explants were transferred on different combination of BAP and Kn with and without adenine sulphate and casein hydrolysate for shoot emergence. Shoot regeneration was observed on MS with 2% sucrose supplemented with 4.0 mg/l Kn, 2.0 mg/l BAP, 10.0 mg/l Adenine sulphate with casein hydrolysate only in case of the callus of cotyledonary explants. For rooting 3-4 cm long shoots were inoculated on ½ MS medium augmented with IBA (0.5, 1.0 and 2mg/l). All rooted plantlets were hardened and successfully transferred to the soil.