Screening for multiple enzymes activities of trichoderma strains isolated from agriculture field soil in senegal

To A total of twenty Trichoderma strains were isolated from rhizospheric soils of tomato fields from different areas of Niaye zone, main area of horticulture production in Senegal. Identification of these Trichoderma isolates was based on morphological and cultural characters on potato dextrose agar medium. These strains were screened for the production of extracellular enzymes such as cellulase, L-Asparginase, chitinase and pectinase. The screening was done by following plate assay method on their respective solid media. Among the 20 strains, 14 strains showed the pectinase activity, 11 strains was positive for L- Asparginase production, 3 strains showed chitinase activity, 15 strains was positive for cellulase activity. The 2 strains (TS1 and NG4) was found to be positive for all the enzymes tested. The excretion of extracellular enzymes reveals their usefulness in the application of Trichoderma species as biocontrol in agricultural soils and in industrial and commercial applications.