Anti inflammatory effect of tea tree oil

Aim : To analyse THE anti-inflammatory effects of tea tree oil Objectives : This research is done to analyse the anti-inflammatory effects of tree tea oil. Background: Many complementary and alternative medicines have enjoyed increased popularity in recent decades.one such product is tea tree oil (tto) which is the essential oil steam distilled from the australian native plant, melaleuca alternifolia. Employed largely for its anti-inflammatory and anti- microbial properties. It is a complex mixture of approximately 100 terpenes and hydrocarbons, the main component being terpinen-4-ol which comprises at least 30% of the oil. Materials and methods: The reaction mixture consists of test extract at different concentrations and 1% aqueous solution of bovine albumin fraction. Ph of the reaction mixture was adjusted using small amount of 1n hcl. The samples were incubated at 37oc for 20 min and then heated at 57oc for 20 min. After cooling the samples, the turbidity was measured spectrophotometrically at 660 nm. The experiment was performed in triplicate. Results: Concentration. The percentage activity was analysed and the results were tabulated. at the concentration of 100 μl, tea tree oil showed significant activity as that of the control.