Betel (Piper betle) is the leaf of a vine belonging to the Piperaceae family, which includes pepper and kava. It is mostly consumed in Asia, combined with tobacco, slaked lime and areca nut and is used as a recreational practise and for oral hygiene. Areca nut extract have been popularly known to exert carcinogenic actions on oral cavity. In this study we have attempted to analyse the effects of betel leaf oil on common periodontal pathogens like Prevotella Intermedia, Porphyromonasgingivalis, Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum. The minimum inhibitory concentration (MIC) required to inhibit the growth of these pathogens was analysed using the TUBE DILUTION METHOD and it was concluded that betel leaf oil has potent antimicrobial actions.