Biochemical characterization and antimicrobial properties of Rhamnolipids produced by pseudomonas fluorescens using mustard Oil as carbon source

Biosurfactants are amphiphilic compounds produced by microorganisms as secondary metabolite. Biosurfactants are microbially produced surface active agents and occur in nature as chemical entities such as glycolipids, phospholipids and lipopeptides. These molecules have attracted considerable scientific attention due to lower toxicity, higher biodegradability. The present study deals with the production and partial purification and characterization of a biosurfactant by Pseudomonas fluorescens. The properties of biosurfactant that was separated by acetone precipitation. The identification of the ability of pseudomonas fluorescens to produce biosurfactants and evaluation their antimicrobial potential are the aim of this work.