Comparative evaluation of sealing ability of endosequence, mta and biodentine as retrograde filling material – an in vitro study

Introduction: The success of periapical surgery is dictated by elimination of infected tissues and adequate apical seal. Among the various materials tested, MTA has shown good sealing ability and biocompatibility in previous studies. Materials like Biodentine, ESBCRRM have been introduced with the aim of overcoming some of the disadvantages of the MTA. Hence the aim of this study was to evaluate the sealing ability of Biodentine, MTA and Endosequence, as a root end filling material, using stereomicroscope. Aim: To Compare and evaluate the sealing ability of MTA, Endosequence and Biodentine as retrograde filling material. Methodology: Sixty extracted maxillary permanent incisors were selected and stored in normal saline. The coronal portion was sectioned at CEJ access cavities were prepared, The working length was determined, BMP was done. Obturation done with gutta-percha. All sample were stored at 37 ± 1°C and 100% relative humidity for 7 days in incubator. The obturated samples were randomly divided into 5 groups.12 samples each group 1-negative control, group 2- positive control, group 3- mta, group 4- endosequence, group 5- biodentine. The apical 3mm of each root were sectioned. Round bur was used to prepare a 3mm root end preparation in all teeth. Apical leakage was evaluated using Rhodamine B dye and measured stereomicroscope. Mean and standard deviation was performed using a one - way ANOVA analysis of variance. Result: There was significantly less microleakage in Group - IV (endosequence) when compared to Group - V (Biodentine) and Group - III (MTA), but there was no significant difference between Group - V (Biodentine) and Group - III (MTA). Conclusion: On comparative evaluation of results of this in vitro study, it was concluded that ES-BCRR, Biodentine& MTA exhibited microleakage with Group II (ES-BCRR) showing the least microleakage of all.