Background: To human health, the significance of Gut Microbiota is highly cherished. The core goal of this research was to do the comparison between the gut microbiota of healthy Chinese and foreign students in Shandong, China. Methods: Fecal samples were collected from 11 healthy Chinese students and from 31 healthy foreign students in Jinan city, Shandong. DNA was extracted, and 456 bp segments comprising hypervariable regions 3 and 4 of the 16S rRNA gene were amplified, barcoded and sequenced. Results: A total of 1,599,407 good quality reads were obtained for evaluating bacterial diversity. Firmicutes was the highest abundant phylum trailed by Bacteriodetes and Actinobacteria. Clostridium constituted the most abundant genus in two groups, but its ratio was higher in foreign students group than Chinese students. The genera Prevotella was in high ratio in foreign students group and genera bacteriodes was in higher ratio in Chinese students group. Although clearvariation between individuals were detected, a huge amount of phylotype is still shared in each group by the healthy microbiota, signifying that a principal microbiome is present in each healthy habitat. However, the diversity and richness showed no difference, the structure of gut microbiota was significantly different between Chinese and foreign students. Conclusion: Phylum Firmicutes and genera Prevotella and Bacteroides constituted the bulk of the human gut microbiota, while there were prominent alterations in composition between the two groups probably because of place, diet and lifestyle.
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