CERTIFICATE

IMPACT FACTOR 2021

Subject Area

  • Life Sciences / Biology
  • Architecture / Building Management
  • Asian Studies
  • Business & Management
  • Chemistry
  • Computer Science
  • Economics & Finance
  • Engineering / Acoustics
  • Environmental Science
  • Agricultural Sciences
  • Pharmaceutical Sciences
  • General Sciences
  • Materials Science
  • Mathematics
  • Medicine
  • Nanotechnology & Nanoscience
  • Nonlinear Science
  • Chaos & Dynamical Systems
  • Physics
  • Social Sciences & Humanities

Why Us? >>

  • Open Access
  • Peer Reviewed
  • Rapid Publication
  • Life time hosting
  • Free promotion service
  • Free indexing service
  • More citations
  • Search engine friendly

Detection of carbapenemase producing klebsiella pneumoniae by phenotypic method

Author: 
Dr. Mohd. Arif, Dr. Dinesh Verma, Dr. A. S. Dalal and Dr. Anamika Vyas
Subject Area: 
Health Sciences
Abstract: 

Introduction: There are several components of normal microbial flora in human intestine and the second most common aerobic bacterial flora is Klebsiella species after Escherichia coli. (Podschum et al., 1998) Klebsiella also accompanies extensive resistance to most of the available antibiotics. Resistance to beta-lactam drugs in gram negative is mainly conferred by beta-lactamase- enzymes that inactivate beta-lactam antibiotics by hydrolysis. (Ghafourian et al., 2011) There for this study was designed to determine Carbapenemase production in Klebsiella pneumonia from various clinical samples by using phenotypic test (Combined disk test, Modified Hodge test, and (combined disk test +Modified Hodge test both). Aim and objectives: The present study was undertaken to determine Carbapenemase production in Klebsiella pneumonia from various clinical samples by using phenotypic test (Combined disk test, Modified Hodge test, and (combined disk test +Modified Hodge test both). Materials and Methods: The presnt study was conducted in department of microbiology, Geetanjali medical college Udaipur (raj). Various clinical samples were obtained from the patients who came in various outdoor and indoor department of Geetanjali medical college Udaipur (raj). 100 non duplicates clinical isolates of Klebsiella pneumonia were processed for the study. Antibiotic susceptibility testing was performed by Kirby bauer method according to CLSI guidelines and the Imipenem resistant isolates were further tested for carbapenemase production by combined-disk test (CDT) and Modified Hodge test (MHT) (Clinical Laboratory Standards Institute (CLSI), 2014) and (combined disk test +Modified Hodge test both). Observation and Results: Among 100 Klebsiella pneumomiae isolates, 14% resistance for Carbepenems (Imipenem) was observed, and 86% Carbepenems (Imipenem) sensitive. all 14 Imipenem resistant Klebsiella pneumoniae isolates, 05 (35.71%) carapenamase positive on CDT; and 04(28.57%) positive by MHT. 03 (21.4)% positive on CDT/MHT whereas 02 (14.28%) were negative on (CDT+MHT) method. Among 14 Imipenem resistant Klebsiella pneumonia isolates, 12(85.71%) were carbapenemase producer and 02(14.2%) were non-carbapenemase producer. Conclusion: To conclude, carbapenemase producing Klebsiella pneumonia isolates were relatively high in our institution. Accurate and timely detection of carbapenemase has important implications for efficient infection control and help in reducing the emergence of resistance thus decreases the morbidity and mortality rate.

PDF file: 

CALL FOR PAPERS

 

ONLINE PAYPAL PAYMENT

IJMCE RECOMMENDATION

Advantages of IJCR

  • Rapid Publishing
  • Professional publishing practices
  • Indexing in leading database
  • High level of citation
  • High Qualitiy reader base
  • High level author suport

Plagiarism Detection

IJCR is following an instant policy on rejection those received papers with plagiarism rate of more than 20%. So, All of authors and contributors must check their papers before submission to making assurance of following our anti-plagiarism policies.

 

EDITORIAL BOARD

CHUDE NKIRU PATRICIA
Nigeria
Dr. Swamy KRM
India
Dr. Abdul Hannan A.M.S
Saudi Arabia.
Luai Farhan Zghair
Iraq
Hasan Ali Abed Al-Zu’bi
Jordanian
Fredrick OJIJA
Tanzanian
Firuza M. Tursunkhodjaeva
Uzbekistan
Faraz Ahmed Farooqi
Saudi Arabia
Eric Randy Reyes Politud
Philippines
Elsadig Gasoom FadelAlla Elbashir
Sudan
Eapen, Asha Sarah
United State
Dr.Arun Kumar A
India
Dr. Zafar Iqbal
Pakistan
Dr. SHAHERA S.PATEL
India
Dr. Ruchika Khanna
India
Dr. Recep TAS
Turkey
Dr. Rasha Ali Eldeeb
Egypt
Dr. Pralhad Kanhaiyalal Rahangdale
India
DR. PATRICK D. CERNA
Philippines
Dr. Nicolas Padilla- Raygoza
Mexico
Dr. Mustafa Y. G. Younis
Libiya
Dr. Muhammad shoaib Ahmedani
Saudi Arabia
DR. MUHAMMAD ISMAIL MOHMAND
United State
DR. MAHESH SHIVAJI CHAVAN
India
DR. M. ARUNA
India
Dr. Lim Gee Nee
Malaysia
Dr. Jatinder Pal Singh Chawla
India
DR. IRAM BOKHARI
Pakistan
Dr. FARHAT NAZ RAHMAN
Pakistan
Dr. Devendra kumar Gupta
India
Dr. ASHWANI KUMAR DUBEY
India
Dr. Ali Seidi
Iran
Dr. Achmad Choerudin
Indonesia
Dr Ashok Kumar Verma
India
Thi Mong Diep NGUYEN
France
Dr. Muhammad Akram
Pakistan
Dr. Imran Azad
Oman
Dr. Meenakshi Malik
India
Aseel Hadi Hamzah
Iraq
Anam Bhatti
Malaysia
Md. Amir Hossain
Bangladesh
Ahmet İPEKÇİ
Turkey
Mirzadi Gohari
Iran