Shortage of water leads to drought stress. Drought stress causes generation of activated oxygen species (AOS) in plants. To overcome water shortage problem plants shows some adaptive mechanism such antioxidant defense and accumulation of osmolytes or osmoprotectants against “AOS”. Activity of enzymes such as catalase (CAT), proxidase (POD) and amylase, protein content and accumulation rate of osmoregulants such as proline and carbohydrate were studied in different Durum Wheay cultivars. The results suggest that water stress increased the activity of enzymes and rate of accumulation of proline and carbohydrate in both cultivars. But out of these two cultivars rain fed cultivar showed significant response during drought stress.