Isolation, characterization, and identification lactic acid bacteria from chicken fish waste faeces that potential as probiotics

The research is aimed at isolating, characterizing, and identifying lactic acid bacteria which have the potential as probiotic agent obtainedfrom the chyme of 34 days-old strain patriot broiler chicken intestine. In order to reach the goal, pour plate and streak plate method on MRS (de Mann Rogosa Sharpe) are used to isolate the bacteria. It is done by adding CaCO3 0.5% as the indicator of the formation of clean zone around the colony. The incubation is conducted at 37ºC for 48 hours. The phenotypic characterization process of the obtained isolates are conducted by gram painting, morphology, catalyst test, temperature influence test, pH and salinity toward the growth, fermentation test, and acid formation test from the carbon source. The identification to find out the possibility of BAL genus and species is conducted through profile matching based on the phenotypic characteristic traced by using Bergey’s Manual of Determinative Bacteriology. Meanwhile, the potential test on its possibility as probiotic agent is conducted by doing the acid resistance test. The production of lactic acid of each isolate of lactic acid bacteria is measured when the bacteria is already 24 hours-old. The result of the research is 15 isolates of lactic acid bacteria. After the confirmation test is conducted, the characteristics show that there are 4 genuses of lactic acid bacteria, i.e. lactobacillus, pediococcus, streptococcus, and enterococcus. The result of the BAL test in producing the lactic acid shows that the AST 04 isolate has the highest amount of lactic acid, 57.69 %, followed by AST 05 which has 9.41 % lactic acid. The BAL isolate of those chicken faces waste still can grow until the concentration of bail salt reaches 0.5 %.