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Isolation, qualitative and quantitative estimation of rna from diabetic and prediabetic subjects

Author:

Talib Yusuf and Talib, S.H.

Subject Area:

Health Sciences

Abstract:

RNA was isolated from Blood samples of various subjects of Diabetes, prediabetes and healthy individual. The entire DNA/RNA that has been extracted was found to differ in their molecular weight and was seen as separate bands when viewed under UV light. The concentration of the RNA was estimated by using the Nanodrop 8000 spectrophotometer. The concentration of healthy subject RNA was found to be more from Diabetic and prediabetic the ratio of their absorbance at 260 and 280 nm, was 2.01 which showed purity in the sample. The concentration of RNA was found to be the least in Prediabetic subject and ratio was found to be 1.89 indicating the presence of contamination. The RNA isolated from the Diabetic having ratio 1.97 which also indicates slight contamination.

PDF file:

30094.pdf

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