This prospective study was carried out on 150 cases having indwelling urinary catheterization for more than 48 hours, to study the microbiological profile of catheter associated urinary tract infections and to find out Antibiotic susceptibility and resistance pattern of various bacterial isolates and ESBL detection from catheter associated urinary tract infection from Dhiraj General Hospital. METHOD: Patients were admitted in Dhiraj General Hospital in various wards and ICU. The study was conducted in 100 patients, whom an indwelling Foley’s catheter was inserted in patients during Catheter Associated Urinary Tract Infection (CA-UTI) and Catheter Associated asymptomatic bacteriuria (CA-ASB) were studied along with microbiological profile and Antibiotic sensitivity patterns of isolated bacteria and ESBL detection during August 2011 to August 2012, in different medical wards, surgery wards and ICU. The catheterized urine sample was collected after catheterization on 5th day onwards on indwelling catheterization. RESULT: Seventy nine per cent of symptomatic catheterized patients showed significant bacteriuria (CA-UTI). Most common pathogen was E.coli (29.4%) followed by Klebsiella spp. (23.16%). Duration of catheterization, Age > 50 and < 12 years, diabetes were associated risk factors. INTERPRETATION AND CONCLUSION: Reducing the use of indwelling catheters, using alternative methods and reducing the duration of catheterization are some of the measures recommended to significantly reduce the consequences thereof CA-UTI in hospitalized patients.