Ecological generation and isolation of heavy metals such as Ag, Au, Cu, etc by Phytoextraction one of the best methods in biotechnology and nanotechnology field for various applications for human use. Since Gold (Au) is one of the expensive heavy metal, so Gold Nanoparticles (GNPs) or compounds are also available in market at a very high price. Therefore the isolation of GNPs by Phytoextraction methods cost comparative very low. GNPs are easily prepared using neem (Azadirachta indica) leaves by phytoextraction procedure using HAucl4. This research work provides a more possible option for generation of GNPs in lesser time, very low cost and non-toxic procedure without using large and complex apparatus. This paper deals with the generation of eco-friendly and cost effective gold nanoparticles by using leaf extract of neem (Azadirachta indica) as reducing agent in short period of time by microwave assistance showing improvement particularly for reduction of time, modulation of size and shape.