The Green Leafy Vegetables (GLVs) such as Brassica juncea (mustard), Trigonella foenum (fenugreek), Amaranthus polygonoides (sirukeerai), Amaranthus tristis (araikeerai) and Sesbania grandiflora (agati) were grown to study their pigment levels in fresh water, 75% of silk dyeing effluent and biotreated effluent conditions as pot study. The pigments such as chlorophyll a, chlorophyll b, Total chlorophyll ab and carotenoids were evaluated on its 45th day of its growth. The total chlorophyll and carotenoids were significantly high in biotreated S.grandiflora and B.juncea. The total chlorophyll level was highly reduced in the T.foenum grown in 75% effluent. In case of carotenoids the most affected plant was S.grandiflora and the least affected was A.tristis grown in 75% effluent. The biotreated B.juncea has an elevated level of carotenoids when compared to other GLVs grown in biotreated effluent.