The comparative evaluation of different bioformulations, viz., vegetative cell application, co-inoculation and co-aggregates application of efficient PGPR cells viz., Pseudomonas fluorescens (PF.3) and Bacillus polymyxa (B.19), together with challenge inoculation of Pyricularia oryzae on the enhancement of induced systemic resistance (ISR) in Rice. Pyricularia oryzae pathosystem was studied under pot culture condition with rice cv. ASD.19. It was observed that the application of Pseudomonas fluorescens and Bacillus polymyxa, as coaggregates, altered the biochemical and physiological parameters viz., reducing and nonreducing sugars, total phenol content and defense enzymes activities such as perocidase (PO), polyphenol oxidase (PPO), of rice plant to a significant livel followed by co inoculation and vegetative cell application of PGPR cells. The application of PGPR cells, as coaggregates, was found to augment the total phenol content and defense enzyme activities such as PO and PPO content of rice plant to a higher level whereas a reduction in reducing and nonreducing sugar level was recorded, which ultimately lead to a reduction of Pyricularia oryzae incidence in upland rice. It has been postulated that the EPS biosynthesis of PGPR cells during co aggregation processes might act as elicitor for the enhancement of ISR in Rice Pyricularia oryzae pathosystem whereas the vegetative cells and coinoculation processes, without any involvement of EPS, responded poorly for the enhancement of ISR in the same pathosystem.