The analysis of potable water quality is important to protect consumers from water-borne or water-based illnesses caused by pathogens like microorganisms, viruses, and protozoa. Rapid identification is critical to ensure water safety. Various detection and identification methods exist; but, they are laborious and time-consuming, so potability confirmation takes longer. This study aims to develop the specific and fast detection of water contamination. We worked on a minimum sample preparation process. In this study, we've got developed a straightforward Paper-based PCR technique. The 16s rDNA primers were used for the detection of microorganism contaminants. LacZ primers were used for coliform detection, which causes serious unhealthiness and hence their detection is crucial for water potability. ITS primers were used for fungal detection. The unique thing about this study is Whatman paper no-1 was used as sample carrier material. We developed and validated the unique paper-based PCR method to detect microbes and coliforms. We evaluated this method for suitability in water potability testing using different water samples.
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