Role of g-csf plus il-15 on neutrophil population in peripheral blood modulating protein tyrosine kinase activity in leukemic mouse

Protein tyrosine kinase (PTK) is an enzyme which mediates cell function, growth, metabolism and apoptosis. The present work has an objective to evaluate the involvement of PTK activity on neutrophil population in the peripheral blood upon stimulation with G-CSF plus IL-15 in leukemic animal. N-N’-Ethyl-nitrosourea (ENU), a carcinogen was used to induce Leukemia in BALB/C mice where in combination with Granulocyte colony stimulating factor (G-CSF) and Interleukin-15 (IL-15) were treated. To determine the PTK activity in normal and leukemic animal universal tyrosine kinase assay kit were used. Appearance of blast cells in peripheral blood and bone marrow smear confirmed the Leukemia. Neutrophils were isolated from spleen using percoll gradient method. Cell population was measured in both normal and leukemic animal before and after treatment of combination of cytokines. ENU induced mice model showed mixed type Leukemia dominating lymphoblastic in nature. The data indicated that treatment with IL-15 and G-CSF in combination to leukemic mice, increased the number of neutrophil population in periphery and under the regulated control of protein tyrosine kinase activity. Results may hint for cell based therapeutic approach to improve neutrophil population in Leukemia.