In the current era, the study of emotional intelligence and psychological well being become a key interest of researcher in various disciplines. This study was undertaken to assess how gender moderate the relationship between emotional intelligence and psychological well-being of adolescents. The study also intends to access the relationship between emotional intelligence and psychological well-being among adolescents. For the purpose of the study, 250 students (125 male and 125 females) enrolled in 11th grade was selected as a sample from the Karad of Satara district. The age range of the students was 16 to 18 years. The data was collected through Emotional Intelligence Test (EII) by Sharma (2011) and Psychological Well-being Scale (PWBS) by Sisodia and Choudhary. Data was statistically analyzed by Pearson Correlational analysis. Study revealed, significant positive correlation between psychological well-being and emotional intelligence among male as well as female adolescents. Additionally, it is found that there is significant relationship between emotional intelligence and psychological well-being and this relationship is moderated by gender. The correlation between emotional intelligence and psychological well-being is higher in female than male adolescents. The present study has great importance which is discussed in the paper.