Aim & Objectives: To assess the role of mastoid process in gender determination by analyzing its dimensions in a computed tomographic image. Methodology: Sample size of 100 CT images was determined, considering a confidence level of 95% and an error rate of +/-5%. Radiographic linear measurements were made using customized DICOM software to accurately analyze the dimensions of mastoids in CT images. Statistics analysis: Z test and Interval estimation was performed. Results: Dimensions of mastoids in CT images of males were found to be higher when compared to females. Conclusion: The results of our study indicated that mastoids are one of the tools for gender identification, yielding a good level of accuracy by using computed tomographic images.