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Significance of CD3 and CD20 in diagnosis of Non-Hodgkin lymphoma in Sudanese patients

Author: 
Yousra Abdelaal Elkhidir Ibrahim and Esam Mohamed Abdul Raheem
Subject Area: 
Health Sciences
Abstract: 

Aim: The aim of this study was to evaluate the significance and importance of CD3 and CD20 in the diagnosis of lymphoma in formalin-fixed paraffin-embedded tissue sections in Sudanese patients. Study design: Cross-sectional descriptive study. Place and Duration of Study: Departments of histopathology at Radiation and Isotopes Center at Khartoum (RICK) and at the National Health Laboratoryduring the period between September 2015 and May 2016. Material and Methods: Tissue sections obtained from 100 formalin-fixed paraffin-embedded tissue blocks of lymphoma lesions were immunohistochemically stained using monoclonal antibodies for CD3 and CD20. Results: CD20 was positive in all B cell lymphomas and negative in all T cell lymphomas, while CD3 is positive in all T cell lymphomas and negative in all B cell lymphomas. Conclusion: Immunohistochemistry is an important tool in diagnosis of lymphoma and differentiation between B cell lymphoma and T cell lymphoma.

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