Stereological investigation of curcumin effects on renal injury induced by diethylnitrosamine experimentally

This investigation has been conducted to study the effect of Curcumin on renal injury induced diethylnitrosamine experimentally in rats. The rats were allocated into four groups each group contain seven animals; Control, Curcumin, Diethylnitrosamine, Curcumin+Diethylnitrosamine. Control group was injected by intra-peritoneal of saline (0.5 ml/kg) for 15 days, curcumin group given by gavage (200 mg/kg/day) for 15 days, diethylnitrosamine group was injected a single dose of diethylnitrosamine at the 5th day (intra-peritoneal, 150 mg/kg), curcumin+ diethylnitrosamine group was given by gavage curcumin (200 mg/kg/day) for 15 days and was injected a single dose of diethylnitrosamine at the 5th day (intra-peritoneal, 150 mg/kg). At end of the experiment, the rats were anesthetized then right kidney was taken for process was followed for light microscopic research. The kidney volume and glomerular number were calculated for each group using physical disector. A reduction of the kidney volume in diethylnitrosamine group and a significant decrease in the number of glomeruli has occurred in diethylnitrosamine group.