To study antibiogram of gram negative septicemia in neonates & obg patients attending maternal & child care hospital at western rajasthan

Background: Neonatal sepsis is a clinical syndrome of bacteremia with systemic signs and symptoms of infection in the first 4 weeks of life1. Most common micro-organisms causing neonatal bacteremia are Escherichia coli, Klebsiellapneumoniae, Staphylococcus aureus, and Group B streptococci3 etc. Bacteremia in Obstetric patients is also a major health concern leading to ICU admission &leades to maternal mortality worldwide 9. Objective: This study was done to analyse gram negative bacteremia and their drug susceptibility pattern among neonates and obstetric patients so that timely appropriate antibiotics therapy be initiated. Methods: This retrospective study was conducted in the Maternal and Child tertiary care hospital from 1 October 2022 to 31 December 2022. Total of 471 suspected case of septicemia were included. Out of 471 patients, 369 (78.34%) patients were pediatrics and 102(21.65%) OBG patients. Blood was collected from suspected cases of bacteremia in Automated blood culture bottles & incubated by automated blood culture system (BD Bactec machine) for 7 days. Positive flagged bottles were processed & species were identified as per standard laboratory protocol. Antimicrobial susceptibility testing was done by Kirby bauer disk diffusion method as per CLSI guideline 2022. Result: 43 (9.12%) bottles were culture positive for gram negative bacteria. Most common GNB isolated were Acinetobacter species (37.20%), Pseudomonas aeruginosa (23.25%), Escherichia coli (18.60%), Klebsiellapneumoniae (16.27%) and showed resistance to Ampicillin (100%) Amoxicillin-clavulanate (93.02%), Ceftriaxone (76.74%), Ceftazidime (76.74%). They were sensitive to Meropenem (100%), Imipenem (88.38%), Amikacin (95.35%), Gentamicin (74.52%). Conclusion: Acinetobacter were the most common isolated pathogen. Routine surveillance of Antimicrobial profile and Antibiotic sensitivity is required to prevent emergence of drug resistance among bacteria.