Ventilator associated pneumonia is a type of hospital acquired infection. It occurs when the patient undergone the ventilator. It develops 48 hrs or longer after mechanical ventilation, by means of an endotracheal tube or tracheostomy. The study was conducted to assess the level of knowledge on prevention of ventilator associated pneumonia among nursing students. The main objectives of study were to assess the knowledge on prevention of ventilator associated pneumonia and to associate the level of knowledge with selected demographic variables. A quantitative research approach with Descriptive research design was used for this study. The target population for the study was GNM students who are studying in selected Nursing Colleges, Kanpur. Sample size was 50 students, were selected by convenience sampling technique and structured questionnaire was used for this study to collect the data. Data analysis was done by using descriptive and inferential statistics on the basis of objectives of the study. The result shows that 20 (40%) having Good knowledge level, 26 (52%) having Average knowledge level and 4 (8%) having Poor knowledge level of students regarding prevention of ventilator associated pneumonia. The maximum mean knowledge score was 12.06, and the Standard Deviation was 5.15. With respect to association between the level of knowledge with the selected demographic variables shows no significance. The study concludes that, the knowledge level of students regarding prevention on ventilator associated pneumonia is average, the study suggest that proper teaching and adequate training regarding prevention on ventilator associated pneumonia will be helpful for the students to gain knowledge.