The present study was conducted to observe the fish biodiversityin Sherudangabeel under Mithapukur Upazilla, Rangpur, Bangladesh during the period fromJanuaryto March 2017. A definite structured questionnaire had used to collect the important data from 50randomly selectedboth temporaryand permanent fishermen from the study areas. The present study had found46 species of fish from the Sherudangabeel. Among the recorded 46 species; four species were highly endangered (8.70%), twenty eightspecies common (60.87%), sevenspecies locally extinct (15.22%) and sevenspecies were rare (15.22%) respectively. Miscellaneous (23.91%) were the most dominant group followed by Catfishes (15.22%), Carps (15.22%), Barbs and Minnows (17.39%), Perches (8.70%), Snakeheads (8.70%), Eels (6.52%) and Clupeids (4.34%) respectively. In the study area species are classified into four types such as endanger (12.77%), vulnerable (6.38%), near threat (6.38%) and least concern (74.47%).This study will be very helpful to indicate areas of high fish biodiversity and make fish sanctuary to reduce the loss of fish biodiversity in the Sherudangabeel.