This study aims to create an alternative media in the form of Chicken Broth Agar (CBA) by utilizing chicken bones. This study succeeded in proving that CBA media could grow more bacteria than NA. This quantitative research is carried out by conducting practical and direct analysis related to the media-making process, and colony analysis in each media. The originality of the research lies in making CBA media in the utilization of chicken bone waste as an effort to increase the variety of bacterial culture media. The limitation of this study lies in the analysis sample which is not representative of all types of microbes. The sample should be adjusted to the composition of the chicken bones.