In vitro anti-microbial activities of aqueous extract of Karappan Kudineer – Siddha formulation

Aim: The aim of this study is to screen In-vitro Anti-microbial activities of aqueous extracts of Karappan Kudineer (KAK) - A Siddha formulation. Methodology: KAK was collected from the pharmacy of ATSVS Siddha Medical College, Munchirai, Kanyakumari Dist. Aqueous extract of KAK was prepared by soxhlet method. In-vitro antimicrobial activity of extract of KAK was screened against Staphylococcus aureus, Streptococcus mutans, Aspergillus niger, Aspergillus flavus and Candida albicans using disc diffusion method. The micro-organisms were collected from the Microbial Type Culture Collection (MTCC). Sterilized discs were soaked in aqueous extract of KAK individually at the concentration of 25mg/disc. Anti-bacterial and anti-fungal suspension was inoculated in Muller-Hinton Agar Media and Potato Dextrose Agar Media respectively. Streptomycin and fluconozole was used as standard drug for the Antimicrobial study. Zone of Inhibition was measured and recorded. Result: Aqueous extract of KAK showed more anti-fungal activity against Aspergillus flavus (11mm) and showed anti-bacterial activity against Staphylococcus aureus (9mm) Conclusion: It is concluded that KAKcan be prescribed as the medicine for skin diseases due to Staphylococcus aureus and Aspergillus flavus infection.