Glucose, glycogen, protein, amino acid and lipid are the major components of the body play an important role in the body construction and energy metabolism. The biochemical estimation were made in the tissues of the fat body, testis, seminal vesicle, vas deferens and male accessory reproductive glands (MARGs). The present study was find out the effect of (phytopesticide) Piperidine on Odontopus varicornis. The insects were exposed to Piperidine for 48h of duration differ significantly, the sub lethal concentration for Piperidine was found to be about 0.15%for 48 hours LD50. The glycogen, protein, lipid content appears to be decreased in the treated insects when compared to the control insects and glucose and amino acid content appears to be increased in the treated insects when compared to the control insects. The values of mean glucose, glycogen, protein, amino acid and lipid content of the fat body, testis, seminal vesicles, vas deferens and MARGs of control and treated insects are compared for significance of difference for which t – values are calculated. The observed t-values are significant at 0.05 level.