Functional groups and compounds analysis using chromatographic and spectroscopic techniques of sesbania grandiflora grown in Untreated and bio remediated silk dyeing effluent

The unique low cost effective technology for the slender entrepreneurs of Silk dyeing effluent has been planned and executed. Environmental outlets like Silk dyeing effluent are harmful and needs a high cost Common Effluent Treatment Plant (CETP) to achieve Zero effluent discharge limits which is not affordable for low investers. In remediating this effluent using the Biofertilizers with multifunctioning activities with Biodegradation, Biocontrol and Plant growth promoting properties were selected. TheGreen leafy vegetable Sesbania grandiflora sowed seeds was treated withraw silk dyeing effluent, Pseudomonas fluorescens and Azospirillum sp. Biofertilizers separately. After 45th days the GLV’s extracts Sesbania grandiflora were grown in fresh water (SGN), Sesbania grandiflora were grown in crude effluent (SGE) and Sesbania grandiflora were grown in biotreated effluent (SGT) were subjected to UV, FTIR and HPLC analysis. Thus from the functional group studies by FT-IR, the alcohol, alkane, alkyl halide and amine groups were found in all the GLV treatments, even in crude effluent, the plants managed to synthesize these organic compounds. The amide group was completely absent in the treatments. The isocyanide group was found only in S.grandiflora grown in fresh water, which was unable to synthesize isocyanide group in plants grown in crude effluent and biotreated effluent. Irrespective of the treatments, the aromatic groups were synthesized by S.grandiflora in biotreated effluent. The HPLC analysis of S.grandiflora grown in fresh water revealed 6 peaks of retention time 2.6, 4.8, 6.4, 15.1, 15.3 and 17.4 minutes of which only 3 peaks were present in S.grandiflora grown in effluent water. In the biotreated plant 4 peaks with the retention time of 2.5, 4.4, 6.6 and 11.2 minutes were found.