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Insilico interaction analysis of herbal bioactive molecules with biofilm associated gene rfaDIN escherichia coli

Author: 
Florida Tilton, Akshaya Puthanpurayil and Lakshmi Priya, K.
Subject Area: 
Life Sciences
Abstract: 

Biofilm is acollection of microbes with a distinct architecture. Biofilms are generally responsible for clogging and corroding pipes, reservoirs, storage area, etc. The quality of household drinking water is also affected by biofilm formation. Biofilms not only cause industrial disasters but are also responsible for causing medical conditions by growing on the surfaces of catheters, heart valve replacements, contact lenses, pacemakers, artificial joints and other surgical implants. Biofilms affect millions of people in the world each year and as a consequence, many deaths occur. Standard antibiotic therapy is often inadequate and the only option may be to remove the contaminated implant. This study was mainly focused on finding novel lead molecules for drug discovery against biofilm associated gene rfaD. The structure of the protein rfaD was modeled using MODELLER. 53 amino acids were chosen in the active site of the protein rfaD using CASTp. Computer aided screening was performed against 54 active compounds from 9 medicinal plants using GOLD. This study provides an organized approach to screen active compounds for the identification of lead molecules for combating biofilm formation in bacteria.

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