Crude methanolic extract of the bark of Mimosa prainiana Gamble (Mimosaceae) was subjected to the preliminary phyto chemical screening and evaluation of its anti inflammatory activity. Preliminary phyto chemical screening of the methanolic extract of the bark of Mimosa prainiana Gamble was followed by HR-LCMS analysis to develop a finger print chromatogram. The studies on the phytochemical screening of the bark extract revealed the presence of flavonoids, phytosterols, carbohydrates and glycosides. Different doses of crude methanolic extract of bark of Mimosa prainiana Gamble was tested for its anti inflammatory activity and found to provide the marked anti inflammatory effect induced by Naja naja venom.