Study on effect of chlorpyrifos on sperm count and head morphology in male mus musculus

Herbicides, Insecticides or pesticides etc may play a role in the development of adverse effects in human and animal bodies. Data concerning its mutagenic and carcinogenic properties have conflicting. In the present study, Effect of Chlorpyrifos on Sperm count and Sperm head abnormalities. Adult Swiss albino mice of the same age group were selected for the research experiment and were divided into three groups (n = 5) consisting of Group C, CPF-1& CPF-2. Group C (Control Group) dosage of 1 ml/per day Distilled water (DW) orally for 6 weeks. Group CPF-1 (Lower dose Group) dosage chlorpyrifos in a dose of 10mg/kg body weight/per day orally for 6 weeks. Group CPF-2 (Higher dose Group) dosage chlorpyrifos in a dose of 20 mg/kg body weight/per day orally for 6 weeks. After the completion of dose duration, the experimental animals were euthanized by cervical dislocation and exposed both caput and cauda epididymis for the analysis of sperm count and sperm head abnormalities. The data obtained from the control and treated group are expressed as Mean% ± SE and ANOVA was used to determine by SPPS Software and the level of significance is p< 0.05. The frequency of sperm head abnormalities was significantly higher than that found in the control group. The frequency of sperm head abnormalities was found 3.09 ± 0.99(Control), 6.92 ± 1.46*(CPF-1), and 14 ± 2.00** (CPF-2) and it is a significant difference between to control and treated groups at p < 0.05. The mean value of the sperm count was 157.02 ± 2.68 (Control), 82.1 ± 2.81* (CPF-1) and 46.5 ± 2.78** (CPF-2). The result show that in both CPF-1 & CPF-2 were significantly decrease the sperm count Hence, results indicate the potential of chlorpyrifos to be gonadotoxic.